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Product Info Summary
| SKU: | M00096-2 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal Antibody, Clone#RM303
View all PDGF R beta Antibodies
SKU/Catalog Number
M00096-2
Size
100uL
Form
Liquid
Description
Boster Bio Anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal Antibody, Clone#RM303 (Catalog # M00096-2). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal Antibody, Clone#RM303 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00096-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM303
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human CD140b
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human CD140b (PDGFR-beta) (Platelet-derived growth factor receptor beta).
Reactive Species
M00096-2 is reactive to PDGFRB in Human
Applications
M00096-2 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
123.968kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:250-1:500 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of human brain tissue lysate using anti-CD140b rabbit monoclonal antibody (Clone RM303) at a 1:100 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue section using anti-CD140b rabbit monoclonal antibody (Clone RM303) at a 1:200 dilution.
Protein Target Info & Infographic
Gene/Protein Information For PDGFRB (Source: Uniprot.org, NCBI)
Gene Name
PDGFRB
Full Name
Platelet-derived growth factor receptor beta
Weight
123.968kDa
Superfamily
protein kinase superfamily
Alternative Names
beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide PDGFRB CD140B, IBGC4, IMF1, JTK12, KOGS, PDGFR, PDGFR-1, PDGFR1, PENTT platelet derived growth factor receptor beta platelet-derived growth factor receptor beta|Activated tyrosine kinase PDGFRB|CD140 antigen-like family member B|NDEL1-PDGFRB|PDGF-R-beta|PDGFR-beta|beta-type platelet-derived growth factor receptor|platelet-derived growth factor receptor 1|platelet-derived growth factor receptor, beta polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDGFRB, check out the PDGFRB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDGFRB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal Antibody, Clone#RM303 (M00096-2)
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No publications found for M00096-2
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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17 Customer Q&As for Anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal Antibody, Clone#RM303
Question
Is there a BSA free version of anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 M00096-2 available?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 M00096-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-02
Question
Is this M00096-2 anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 reactive to the isotypes of PDGFRB?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
The immunogen of M00096-2 anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 is A peptide corresponding to the C-terminus of human CD140b. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-26
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 M00096-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-21
Question
I am interested in using your anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 for positive regulation of rho protein signal transduction studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
We appreciate your inquiry. This M00096-2 anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 is tested on human kidney tissue, brain tissue, tissue lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-25
Question
We need to test anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 M00096-2 on human brain for research purposes, then I may be interested in using anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 M00096-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Miller
Verified customer
Asked: 2019-03-26
Answer
The products we sell, including anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 M00096-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-26
Question
We purchased anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone
A. Carter
Verified customer
Asked: 2018-12-27
Answer
I looked at the website and datasheets of our anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2018-12-27
Question
Would anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 M00096-2 work for WB with brain?
Verified Customer
Verified customer
Asked: 2018-11-15
Answer
According to the expression profile of brain, PDGFRB is highly expressed in brain. So, it is likely that anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 M00096-2 will work for WB with brain.
Boster Scientific Support
Answered: 2018-11-15
Question
We are currently using anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 M00096-2 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-15
Answer
The anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 (M00096-2) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-15
Question
Is a blocking peptide available for product anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 (M00096-2)?
Verified Customer
Verified customer
Asked: 2018-10-15
Answer
We do provide the blocking peptide for product anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 (M00096-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-10-15
Question
Does anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 M00096-2 work on primate WB with brain?
S. Li
Verified customer
Asked: 2018-08-13
Answer
Our lab technicians have not tested anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 M00096-2 on primate. You can run a BLAST between primate and the immunogen sequence of anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 M00096-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-13
Question
Here is the WB image, lot number and protocol we used for brain using anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 M00096-2. Please let me know if you require anything else.
S. Patel
Verified customer
Asked: 2018-04-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-03
Question
Does M00096-2 anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-28
Answer
You can see on the product datasheet, M00096-2 anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-28
Question
My question regarding product M00096-2, anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-15
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00096-2 anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-15
Question
I was wanting to use your anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2017-07-21
Answer
As indicated on the product datasheet, M00096-2 anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-21
Question
My boss were happy with the WB result of your anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2017-05-11
Answer
From literature, brain does express PDGFRB. Generally PDGFRB expresses in cell membrane. Regarding which tissues have PDGFRB expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-05-11
Question
I see that the anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 M00096-2 works with WB, what is the protocol used to produce the result images on the product page?
B. Taylor
Verified customer
Asked: 2017-01-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-01-27
Question
We have observed staining in human brain. Do you have any suggestions? Is anti-CD140b (C-term) (PDGFR-beta) (Platelet-derived growth factor receptor beta) Rabbit Monoclonal antibody, Clone#RM303 supposed to stain brain positively?
B. Mangal
Verified customer
Asked: 2013-11-20
Answer
According to literature brain does express PDGFRB. According to Uniprot.org, PDGFRB is expressed in ectocervix, brain, among other tissues. Regarding which tissues have PDGFRB expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-11-20
